ORF45 colocalizes with trans-Golgi network but K297R mutants does not in the context of virus.

<p>Cells carrying BAC-cloned KSHV (iSLK-BAC16) and ORF45-K297R mutant recombinant virus (iSLK-BAC-K297R) were induced with Dox (3 μg/ml) for lytic KSHV replication. Three days post-induction, cells were stained with mouse monoclonal anti-ORF45 (green) and rabbit polyclonal anti-TGN46 (red). The nuclei were stained by Hoechst (blue). The white boxes within the merged panels are shown as enlarged pictures for the details of colocalization.