Q6 could induce DNA DSBs followed by G2/M arrest in hypoxia.

<p>A. Western blot was employed to assess the DNA DSBs induced by Q6, TPZ, VP16. Protein levels of γ-H2Ax were detected, and β-Actin was measured as the loading control. Data are representative of three independent experiments. B. Immunofluorescene staining was employed to detect the formation of γ-H2AX foci in HepG2 cells. C. and D. Q6 induced G2/M arrest in hepatocellular carcinoma cells. Cells treated with Q6 (1.25 μM, 2.5 μM and 5 μM) or TPZ (10 μM, 20 μM) for 24 h under hypoxia (1% O₂) condition. Then the cells were collected and prepared for cytometric analysis of cell cycle distribution. Representative cell cycle distribution figures were labeled. The percentages of the cell population in different phases of cell cycle were analyzed by CELL Quest.