CP190-L is important for spindle formation in developing brain.

<p><i>Drosophila</i> NBs were fixed and stained as indicated. <i>A</i>. Metaphase NBs are shown with CP190 in red, Asl to mark the centrosome in green, and DAPI in blue. WT control (<i>cp190</i>^<i>ΔL</i><i>/TM6</i>) is in the top row and mutant <i>cp190</i>^<i>ΔL</i><i>/ Df</i>^<i>p11</i> in the bottom row. Boxed regions indicating centrosomes are magnified and shown on the far right panels (numbers next to centrosome indicate which zoomed centrosome is displayed). Yellow arrows point out centrosomes in the merged channel. White dotted line indicates NB outline. <i>B</i>.Fixed anaphase NBs. Labeling is the same as in <i>A</i>. Note a lagging chromosome in <i>cp190</i>^<i>ΔL</i><i>/ Df</i>^<i>p11</i> (yellow arrowhead), which is never seen in WT (frequency indicated in DAPI channel). Scale bar for <i>A</i> and <i>B</i> = 5μm, zoom = 1μm. <i>C</i>, Live imaging of MTs in WT (<i>cp190</i>^<i>ΔL</i><i>/TM6</i>) and <i>cp190</i>^<i>ΔL</i><i>/ Df</i>^<i>p11</i>mutant NBs, note bent spindle (frequency indicated in right most image) and detached centrosome (yellow arrows) in mutant cell. Scale bar = 5μm. Panels in C from top to bottom correspond to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144174#pone.0144174.s007" target="_blank">S1 Movie</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144174#pone.0144174.s008" target="_blank">S2 Movie</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144174#pone.0144174.s009" target="_blank">S3 Movie</a>, respectively.