4H3MC attenuates T cell proliferation during T cell differentiation.

<p>(A) CD4⁺ T cells from LNs and spleens were differentiated into Th1- or Th2-polarized cells, as described in Materials and Methods, in the absence or presence of 4H3MC (10 μM) for 5 days. On Day 5, cells were harvested and total cell numbers measured. (B) CD4⁺ T cells from LNs and spleens were labeled with CFSE (10 μM) for 30 min and differentiated in the absence or presence of 4H3MC. After 72 h of culture, cells were harvested and the CFSE intensity measured by flow cytometry. 0 h indicates naïve CD4⁺ T cells. The numbers above 72 h population indicates the number of cell division. Data are representative of three independent experiments. Data are expressed as the mean ± SD from three independent experiments. *P < 0.05, <i>versus</i> the cell not treated with 4H3MC.