Transient subgenomic HCV replicon assays in duplicates.

<p>RLU—Relative Light Unit. The luciferase activity was measured in cell lysates at 4, 24, 48 and 72 h after transfection of RNA transcripts from pSGR-luc-JFH1 (WT), pSGR-Luc-W501R-JFH1 (W501R) and pSGR-Luc-JFH1/GND (GND). Data were normalized for luciferase activity values at 4 h. Bars represent the mean of two independent experiments with variability below 15%.