Lyoprotectants are stabilizers used to prevent denaturation of proteins during freeze-drying and subsequent storage. In order to be effective, lyoprotectants must be retained amorphous. The physical state of the lyoprotectant is usually characterized by powder X-ray diffractometry of the dried cake. While trehalose is widely used as a lyoprotectant, we report its crystallization during freeze-drying and point out why it may not become evident from characterizing the final lyophile. When an aqueous trehalose solution was cooled to −40 °C, ice was the only crystalline phase observed. However, upon annealing at −18 °C, crystallization of trehalose dihydrate was evident. During drying, the dihydrate dehydrated to substantially amorphous anhydra...
As a lyophilized product, Saccharomyces boulardii has been commonly used to treat diarrhea in adults...
Lyophilization is a process often used to stabilize labile molecules in the solid-state. The process...
AbstractDisaccharides are well-known reagents to protect biostructures like proteins and phospholipi...
International audienceThe freezing step influences lyophilization efficiency and protein stability. ...
Effect of lyoprotectants on the survival rate of freeze-dried lactic acid bacteria was investigated....
Purpose To study how water plasticization affects the molecular mobility and crystallization tendenc...
There is a lack of methods to predict the isothermal crystallization behavior of amorphous freeze-dr...
peer-reviewedIn recent years, freeze drying of protein preparations above the collapse temperature o...
Freeze-drying, or lyophilization, is the most commonly used technique for the preservation of protei...
Trehalose forms a glass that can be used to preserve labile substances under desiccation. The crysta...
AbstractA study is presented of the role of cholesterol content on the gel-to-liquid crystalline pha...
Simple sugars, especially disaccharides, stabilize biomaterials of various composition during air-dr...
Problem - The primary objective of this dissertation was to elucidate the protective effects and mec...
The collapse temperature (Tc) and the glass transition temperature of freeze-concentrated solutions ...
Understanding of how the stabilization mechanism of trehalose operates on biological molecules again...
As a lyophilized product, Saccharomyces boulardii has been commonly used to treat diarrhea in adults...
Lyophilization is a process often used to stabilize labile molecules in the solid-state. The process...
AbstractDisaccharides are well-known reagents to protect biostructures like proteins and phospholipi...
International audienceThe freezing step influences lyophilization efficiency and protein stability. ...
Effect of lyoprotectants on the survival rate of freeze-dried lactic acid bacteria was investigated....
Purpose To study how water plasticization affects the molecular mobility and crystallization tendenc...
There is a lack of methods to predict the isothermal crystallization behavior of amorphous freeze-dr...
peer-reviewedIn recent years, freeze drying of protein preparations above the collapse temperature o...
Freeze-drying, or lyophilization, is the most commonly used technique for the preservation of protei...
Trehalose forms a glass that can be used to preserve labile substances under desiccation. The crysta...
AbstractA study is presented of the role of cholesterol content on the gel-to-liquid crystalline pha...
Simple sugars, especially disaccharides, stabilize biomaterials of various composition during air-dr...
Problem - The primary objective of this dissertation was to elucidate the protective effects and mec...
The collapse temperature (Tc) and the glass transition temperature of freeze-concentrated solutions ...
Understanding of how the stabilization mechanism of trehalose operates on biological molecules again...
As a lyophilized product, Saccharomyces boulardii has been commonly used to treat diarrhea in adults...
Lyophilization is a process often used to stabilize labile molecules in the solid-state. The process...
AbstractDisaccharides are well-known reagents to protect biostructures like proteins and phospholipi...