Levels of IFNγ (A) and IL-4 (B) in the supernatants from splenic cultures of N-PmpC EcN BGs-, EcN BGs- and non-immunized BALB/c mice.

<p>Mice were immunized subcutaneously (s.c.//) or via the conjunctiva (conj//). Each group consisted of 10 mice. Splenocytes were cultivated at 37°C in 5% CO₂ for 48 h in 10% FCS/RPMI 1640/50 μM β-mercaptoethanol supplemented with 10 μg/ml N-PmpC (+) or without any additional supplementation (-). Differences in concentrations of cytokines in culture supernatants were evaluated using a 1-way repeated ANOVA (<i>p <0</i>.<i>05*</i>, p <i><0</i>.<i>005**</i>_,<i>p < 0</i>.<i>0001***</i>). Corresponding (non-stimulated and N-PmpC-stimulated) non-immunized group cultures were used as a reference unless otherwise indicated by two head-arrow.