Inflammasome components can be linked to PFT-induced necroptosis in macrophages.

<p>LDH release assay of BMDM from C57BL/6 wildtype (WT), NLRP3 KO, ASC KO and MyD88 KO mice, infected with <b>A)</b><i>S</i>. <i>marcescens</i> (<i>Sma</i>; MOI = 0.5), <b>B)</b><i>S</i>. <i>aureus</i> (<i>Sau</i>; MOI = 10), <b>C)</b> UPEC (MOI = 100), <b>D)</b><i>S</i>. <i>pneumoniae</i> (Spn; MOI = 100) and challenged with <b>E)</b> recombinant pneumolysin (rPly; 100 μg/ml). <b>F)</b> Levels of IL-1β detected by ELISA in supernatants from MH-S macrophages infected with <i>Sma</i> or Δ<i>shlA</i>. Necrostatin-5 (Nec-5) was added at 100μM. <b>G)</b> Levels of IL-1β detected in supernatants from WT, NLRP3 KO, ASC KO BMDMs infected with <i>Sma</i>, <i>Sau</i>, UPEC and <i>Spn</i> and uninfected controls. For multiple group comparisons Dunn’s multiple-comparison post-test was used: *, <i>P</i> ≤ 0.05. Data are representative of ≥3 separate experiments, each with 8 biological replicates.