Inhibition of EPS-induced cell wasting in C2C12 myotubes by Nrf2 activation.

<p>(a) Images show immunocytochemical staining of Annexin V and propidium iodide (PI) staining in EPS-treated and untreated C2C12 myotubes. Annexin V positive signals are indicated with arrows. (b) The graph shows fluorescence intensity of Annexin V/HPF in C2C12 myotubes. Annexin V expression was measured by fluorescence microscopy imaging (n = 6). (c) Graph shows the cell viability of non-EPS or EPS treated C2C12 myotubes (n = 6) as determined by the MTT assay. *p < 0.001 vs. mock-transfected; †p < 0.05 vs. non-EPS.