Analysis of IFNG expression in CD3+ and CD3- cell subsets.

<p>PBMC from LTBI+ donors were stimulated with PPD or left unstimulated, stained with antibodies against surface markers as indicated, probed with Cy5-labeled IFNG probes, and analyzed by flow cytometry. Gates were set based on unstimulated samples and Fluorescence Minus One (FMO) controls. The scatter plots show data from one donor and the bar graphs from three donors. In each panel, frequencies were calculated relative to the total number of cells in the panel. (a) Frequencies of IFNG+CD3+ cells (left panel) and IFNG+ cells in the CD4+, CD8+ and γδ T cell subsets (right panel). (b) Frequency of CD4+, CD8+, and γδ subsets in IFNG+CD3+ cells. (c) Frequencies of IFNG+CD3- cells (left panel) and IFNG+CD56+ cells (right panel, upper right quadrants). (d) Frequency of CD56 in IFNG+CD3- cells.