Dysregulated insulin signaling but unchanged PrP^C expression in HFD-fed mouse brains.

<p>(<b>A</b>) Left: Western blot of p^Ser473-Akt and total Akt in ND- or HFD-fed mouse brains. Right: densitometric quantification of the Western blot showed significantly lower p^Ser473-Akt/Akt ratio in HFD-fed mouse brains (n = 3, *, <i>p</i> = 0.0280) (<b>B</b>) Left: Western blot of p^Ser9-GSK3β and total GSK3β in ND- or HFD-fed mouse brains. Right: densitometric quantification of the Western blot revealed a trend of reduced lower p^Ser9-GSK3β/ GSK3β ratio in HFD-fed mouse brains (n = 3, n.s <i>p</i> = 0.0890). (<b>C</b>) qRT-PCR of <i>Prnp</i> expression in HFD-fed and ND-fed mouse brains showed similar levels of <i>Prnp</i> mRNA (n = 3, n.s <i>p</i>>0.05). (<b>D</b>) Left: Western blot of PrP^C in HFD-fed and ND-fed mouse brains. Right: densitometric quantification of the Western blot demonstrated a similar level of PrP^C expression (n = 3, n.s <i>p</i>>0.05). <b>(E)</b> ELISA of PrP^C showed similar level of PrP^C expression in HFD-fed and ND-fed mouse brains (n = 3, n.s <i>p</i>>0.05).