PAK1 activity is important for Nischarin-regulated neurite outgrowth in Neuro-2a cells.

<p><b>(A)</b> Representative images of Neuro-2a cells transfected with Nis-shRNA-3 and treated with the PAK1 inhibitor IPA3. Scale bar: 20 μm. <b>(B)</b> Neuro-2a cells were transfected with Nis-shRNA-3 and treated with IPA3. Phosphorylation of PAK1/2 was determined by western blot assay. IPA3 treatment reversed the increases in the number of neurite-bearing cells <b>(C)</b>, the mean length of the longest neurite <b>(D)</b>, and total length of neurite per cell <b>(E)</b> in Nischarin-suppressed cells. Asterisks indicate significant differences between ctl-shRNA and Nis-shRNA (***<i>p</i><0.001, n = 3), or between Nis-shRNA and Nis-shRNA+IPA3 (^#<i>p</i><0.05, ^##<i>p</i><0.01, n = 3). Data presented are the mean ± SEM.