Live 0ΔNLS and UV-0ΔNLS vaccines activate peripheral CD8⁺ T-cells at a comparable frequency in C57BL/10 mice.

<p><b>(A)</b> Design of vaccine-challenge studies. Mice received footpad immunizations on Days 0 and 30 with culture medium (mock), U.V-0ΔNLS, or 2 x 10⁶ pfu live 0ΔNLS, and blood was collected on Day 7 post-boost to analyze CD11a and CD43 activation marker expression on CD8⁺ T-cells. <b>(B)</b> Within peripheral WBCs, a primary gate was set on cells with the forward- and side-scatter properties of mononuclear cells, and a secondary gate was set on CD8⁺ Thy1.2⁺ T-cells, which were analyzed for the frequency of cells expressing elevated levels of CD11a and an activation-associated glycoform of CD43. <b>(C and D)</b> Mean ± sem frequency of CD11a^hi, CD43^hi CD8⁺ T-cells in peripheral blood of mice on <b>(C)</b> Day 0 and <b>(D)</b> Day 7 post-boost (n = 16 per group per time). A double asterisk (**) denotes p < 0.001 that the frequency of CD11a^hi, CD43^hi CD8⁺ T-cells was equivalent to mock-immunized control mice on that day, as determined by one-way ANOVA and Tukey's post-hoc-test.