Zymogram of gelatinolytic activities.

<p>(A) Zymogram of the gelatinolytic activities of the HT 1080 cell fraction (C; 13.7 mU of cathepsin L activity) and culture medium (M; 4.33 mU of cathepsin L activity). The activities were estimated in the presence (+) or absence (−) of 0.1 mM leupeptin. The gel and gelatin concentrations were 11.5% and 0.8 mg/mL, respectively. The following molecular markers were used: 116.0 kDa, β-galactosidase; 97.4 kDa, phosphorylase b; 66.0 kDa, BSA; 45.0 kDa, ovalbumin; and 31.0 kDa, carbonic anhydrase. (B) Zymogram of the gelatinolytic activities of the HT 1080 cell fraction (C), lysosomal fraction (L), and culture medium (M), obtained by using the improved gelatin zymography technique with leupeptin.