Y-27632 promoted in vivo corneal epithelial wound healing.

<p>Corneal epithelial wounds were created in both eyes of Sprague Dawley rats. Y-27632 or PBS was applied topically five times a day to the left or right eye, respectively. (A) After wounding, the area of epithelial defect in each cornea was revealed by staining with 1% fluorescein, and photographed every 24 hours until healed. Representative photographs of corneal healing in five different experiemnets were shown here. The corneal epithelial wounds were rapidly healed in Y-27632-treated eyes. (B) The area of the photographed corneal epithelial defect was measured by a computer-assisted digitizer and statistical analyses were performed using a paired test, and *p<0.05 was considered significant (n = 5, p = 0.02 and 0.002 at day 2 and day 3, respectively). (C). Healed corneas were fixed and stained with anti-Ki67 antibody. Ki67 was more abundantly expressed in the Y-27632-treated corneas, confirming that proliferation was actually increased by Y-27632. The nuclei were counterstained by DAPI (blue). Scale bar, 100 μm. Magnification: 200×.