MOESM9 of Leaf-residing Methylobacterium species fix nitrogen and promote biomass and seed production in Jatropha curcas

Additional file 9: Figure S6. Gas chromatography chromatogram showing ethylene and acetylene peaks. (a) 0.5 ml of 4.85 μmol ethylene (C2H4, Product Number: 00489, Sigma-Aldrich) standard was injected in GC. (b) Strain L2-4 inoculated in N-free medium (40 ml) and ARA was performed by injecting purified acetylene into the bottles sealed with gas-tight serum stoppers to yield 15 % acetylene (v/v); this was followed by incubation for up to 48 h at 30 °C. (c) ARA was performed without strain L2-4 (blank)