TSP50-o/e cells induced Inflammation, macrophage infiltration and M2b polarization in vivo.

<p>(A) Images of all the nude mice from each group (n = 8) at the end of the experiments. (B) Photographs of the dissected tumors from each group. (C) Tumor weight of each group. (D) Images representing the FACS data showing the quantification of CD11b and F4/80 macrophages infiltrated in TSP50-o/e tumors or the control tumors. The experiment was repeated three times with three to four mice in each group and similar results were obtained. (E) Quantitative PCR analysis of mRNA for M1- and M2-specific genes in the macrophages sorted from TSP50-o/e tumors or the control tumor. Data are shown as mean ± SD of three independent experiments. Statistical significance is assessed by a two sample t-test, where *denotes P<0.05, and ** denotes P<0.01.