Genetic analysis of sporulation, spore viability, and recombination in various <i>zip1</i> diploids.

<p>(A) <i>ZIP1</i> (NHY957), <i>zip1-4D</i> (NH2241::p382), <i>zip1Δ</i> (NH2241::pRS304), and <i>zip1-4A</i> (NH2241::p382-4A) were sporulated for 2 d at 30°C. Average sporulation from five independent colonies was graphed; error bars indicate the standard deviation. (B) Average spore viability calculated from dissection of nine independent transformants from each diploid. Brackets indicate strain pairs whose spore viability is significantly different (χ²; <i>p</i> < 0.0001). The number of tetrads dissected for each strain: <i>ZIP1</i>, 449; <i>zip1-4D</i>, 1,332; <i>zip1-4A</i>, 1,857; and <i>zip1Δ</i>, 4,012. The isogenic <i>ZIP1</i> data shown in Panels B and E were previously published [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002329#pbio.1002329.ref035" target="_blank">35</a>]. (C) Map distances for the indicated intervals on Chromosomes III, VII, and VIII were calculated using the formula from Perkins [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002329#pbio.1002329.ref036" target="_blank">36</a>]. At least 940 tetrads were analyzed for each genotype. <i>ZIP1</i> map distances were generated by pooling data from published NHY957 dissections [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002329#pbio.1002329.ref025" target="_blank">25</a>,<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002329#pbio.1002329.ref035" target="_blank">35</a>,<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002329#pbio.1002329.ref037" target="_blank">37</a>]. <i>p</i>-Values indicating the significance between the map distances of different intervals for mutants and WT are shown in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002329#pbio.1002329.s002" target="_blank">S1 Data</a>. (D) The percent of four viable spore tetrads exhibiting non-2⁺:2⁻ segregation (gene conversion) for the indicated loci. <i>ZIP1</i> data were taken from [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002329#pbio.1002329.ref037" target="_blank">37</a>]. (E) The distribution of viable spores in tetrads from the dissections shown in Panel B. (F) Interference values for adjacent intervals were calculated using the method of [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002329#pbio.1002329.ref038" target="_blank">38</a>]. A solid line indicates interference was observed when either interval was used as the reference. A black broken line indicates interference was detected using one of the reference intervals. A red broken line indicates that no interference was observed using either reference interval. Numbers represent the average of the two ratios for that interval. Data for the <i>ZIP1</i> strain in Panel F are from [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002329#pbio.1002329.ref037" target="_blank">37</a>]. The aberrantly high value observed for the <i>zip1Δ</i> in the <i>HIS4-LEU2/LEU2-CEN</i> interval is likely due to the chance occurrence of an extra nonparental ditype (NPD).