Dynamic patterns of H3K4me2/3 during porcine IVF and SCNT cleavage-stages embryo development.

<p><b>(A)</b> Representative images of IVF and SCNT embryos at different developmental stages immunostained with an anti-H3K4me2 antibody. Antibody was localized with an Alexa Flour 488-conjugated secondary antibody (green). DNA was stained with propidium iodide (red). Middle panels showed the merged images (yellow) between H3K4me2 signal (green) and DNA staining (red). Red dash line marks the developmental stages with different H3K4me2 signal intensity between IVF and SCNT embryos. Arrow denotes H3K4me2 signal intensity was abnormally higher in SCNT embryos than that in IVF counterparts. Arrowhead means H3K4me2 signal intensity was abnormally lower in SCNT embryos than that in IVF counterparts. <b>(B)</b> Representative images of IVF and SCNT embryos at different developmental stages immunostained with an anti-H3K4me3 antibody. Arrow denotes H3K4me3 signal intensity was abnormally higher in SCNT embryos than that in IVF counterparts. Scale bar = 50 μm. <b>(C)</b> Quantification of H3K4me2 intensity between IVF and SCNT early embryos. (D) Quantification of H3K4me3 intensity between IVF and SCNT early embryos. Blue bars denote IVF group, red bars represent SCNT group. Values are mean ± S.E.M. Different letters (a-b) on the bars indicate a statistically significant difference between IVF and SCNT groups (<i>p</i> < 0.05).