Binding of buffalo portal-round three selected (Bp-R3) single-chain antibody Fv domain (scFv) phage panned against infected mouse sera and scFv-alkaline phosphatase (scFv-AP) fusion proteins to <i>Schistosoma japonicum</i> soluble whole adult preparation (SWAP) by ELISA.

<p>Data represent mean ± S.E.M. of the O.D._450nm for Bp-R3-A and ES pooled phage libraries (Bp-R3-AES) and Bp-R3-post-infected-mouse-sera (Bp-R3-PIMS) phage binding to <i>S</i>. <i>japonicum</i> SWAP (A), and scFv-AP proteins (Bp-scFv-1 to Bp-scFv-5) binding to SWAP (B and C) or SWAP spiked naive mouse sera (D). Antibodies derived from an <i>S</i>. <i>japonicum</i> infected rabbit (infected antibodies) were used as a positive control (D#). Statistical significance was determined by one- or two-way ANOVA followed by Tukey’s post-hoc test, where * <i>P</i> < 0.05, *** <i>P</i> < 0.001, **** <i>P</i> < 0.0001.