Silencing of <i>IRS2</i>, <i>CUL4A</i> and <i>TFDP1</i> in iCCA cells.

<p>(a) RBE cells were transiently transfected with siRNAs (RBE-siIRS2, RBE-siCUL4A and RBE-siTFDP1). The effect of gene expression after manipulation on growth potential was determined by Western blotting using anti-PCNA antibody (left) and manual counting of total cell numbers (right). (b) Silencing of the target genes, which were confirmed by Western blotting, dramatically reduced the mobility potential of RBE cells. Data represent means ± standard deviations for three independent experiments.