DOG1_Col is a natural non-binding weak allele.

<p>(<b>A</b>) Germination profiles of Col, L<i>er</i> and NIL DOG1 after different periods of dry storage. Pink triangle, Col; green open diamond, L<i>er</i>; and blue square, NIL DOG1. Error bars represent S.E.M. of at least three biological replicates. w, week. (<b>B</b>) <i>DOG1</i> overall mRNA and protein levels in L<i>er</i>, NIL DOG1 and Col. Details are described in the legend of <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005737#pgen.1005737.g002" target="_blank">Fig 2E and 2F</a>. Numbers below the blot indicate relative DOG1 protein levels, normalized to the loading control. Error bars represent S.E.M. of at least three biological replicates. The data for L<i>er</i> and NIL DOG1 were taken from [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005737#pgen.1005737.ref018" target="_blank">18</a>] (<a href="http://www.plantcell.org" target="_blank">www.plantcell.org</a>): Copyright American Society of Plant Biologists. (<b>C</b>) Yeast two-hybrid binding assay of the beta isoforms of DOG1_Col and controls. Details are described in the legend of <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005737#pgen.1005737.g004" target="_blank">Fig 4A</a>. (<b>D</b>) Alignment of the first 20 amino acid residues of DOG1 of L<i>er</i>, Cvi and Col. The first M is the starting methionine. Polymorphic residues are shaded in grey, and the asterisk represents the 16^th tyrosine_L<i>er</i> that is required for self-binding. (<b>E</b>) The ECCY substitution of DOG1_Col shows enhanced dormancy. Germination profiles upon harvest of transgenic lines with ECCY substitution and controls. Col WT represents 14 independent lines of <i>dog1-2</i> transformed with wild-type Col_<i>DOG1</i>, Col ECCY represents 13 independent lines of <i>dog1-2</i> transformed with the ECCY variant of Col_<i>DOG1</i>. Error bars represent S.E.M.