Connections between cells in collective outgrowth events are mediated by VE-cadherin junctions.

<p>Samples were prepared as described for Figs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145210#pone.0145210.g005" target="_blank">5</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145210#pone.0145210.g006" target="_blank">6</a>, they were fixed with formaldehyde and embedded in collagen gels prior to immunostaining and confocal imaging. HUVEC (A-F) or LEC (G-L) pairs from collective outgrowth events on Fn fibers, in the presence (A-C for HUVEC and G-I for LEC) or absence (D-F for HUVEC and J-L for LEC) of 50 ng/ml VEGF-A are shown stained with DAPI (A, D, G, J), anti-VE-cadherin (B, E, H, K) and phalloidin (C, F, I, L). Scale bar: 10 μm.