Effect of macrophage depletion in <i>Ccr6^-/-</i> mice.

<p>(A) Representative F4/80 immunostaining of liver sections of wt and <i>Ccr6</i>^<i>-/-</i> mice injected with PBS liposomes (vehicle) (n = 8) or clodronate liposomes (n = 13) followed by CCl4 acute liver injury (see <i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145147#pone.0145147.s006" target="_blank">S1 Text</a></i>). (B) Representative H&E staining of liver sections (x100 magnification) of wt and <i>Ccr6</i>^<i>-/-</i> mice injected with clodronate liposomes followed by CCl4 acute liver injury (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145147#pone.0145147.s006" target="_blank">S<i>1 Text</i></a>). (C) <i>Icam1</i>, <i>Il6</i>, <i>Tnf-α</i>, <i>Nos2</i>, <i>Mcp1</i> and <i>Col1a1</i> hepatic gene expression in <i>Ccr6</i>^<i>-/-</i> (KO) mice injected with PBS liposomes or clodronate liposomes in presence (1xCCl4–injected) or absence (vehicle, corn-oil injected) of CCl4-induced acute liver damage (see <i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145147#pone.0145147.s006" target="_blank">S1 Text</a></i>) (*p<0.05). (D) <i>Icam1</i>, <i>Il6</i>, <i>Tnf-α</i> and <i>Col1a1</i> hepatic gene expression in wt and <i>Ccr6</i>^<i>-/-</i> (KO) mice injected with clodronate liposomes and vehicle- (corn oil) or 1xCCl4 (*p<0.05 with respect to wt). (E) ALT, AST and LDH serum levels in wt and <i>Ccr6</i>^<i>-/-</i> (KO) mice in the presence (PBS liposomes injected) and absence (clodronate liposomes injected) of macrophages in animals with CCl4-induced acute liver injury (*p<0.05).