Proximal promoter region of <i>DUSP5</i> contains a functional serum responsive element.

<p>(A) DNA sequences of <i>DUSP5</i> promoter region containing proximal CArG box (CArG2) with proximal EBS and unrelated probe used in Electrophoretic Mobility Shift Assays (EMSA). (B) EMSA was performed with the end labelled probe containing wild type CArG Boxe and EBS using 5 μg of nuclear extracts isolated from NIH/3T3 transfected with hemagglutinin (HA) tagged Elk-1expression plasmid. For competition experiments, different unlabelled oligonucleotides (lane 3 to 7) were used. The indicated antibodies (lane 8 to 10) were used for supershift analysis. (C) EMSA was performed with the end labelled probe containing wild type CArG Boxe and mutated EBS using 5 μg of the same nuclear extracts. The indicated antibodies (lane 3 to 5) were used for supershift analysis.