Additional file 7: of Dysregulation of RasGRP1 in rheumatoid arthritis and modulation of RasGRP3 as a biomarker of TNFα inhibitors

TNFα has no effect on T cell apoptosis. After T cell negative selection, cells were cultured with or without TNFα for 48 and 72 hours. To measure apoptosis, a fluorescein isothiocyanate (FITC) annexin-V Apoptosis Detection Kit (BD Biosciences, USA) was used. After labeling with FITC annexin-V and propidium iodide (PI), cells were analyzed by flow cytometry within 1 hour. a One representative result of three independent experiments is presented. b Histogram represents the mean ± standard error of the mean (SEM) of percentage of annexin-V positive cells in three independent experiments. Mean ± SEM were compared using Student’s t test. (TIF 1318 kb