Additional file 8: Figure S6. of A kinome siRNA screen identifies HGS as a potential target for liver cancers with oncogenic mutations in CTNNB1

HCT116WT/S45del and HCT116WT/- cell growth in response to knockdown of potential lethal synthetic hits HGS; STRADA, FES, BRAF and PKMYT1. HCT116WT/S45del and HCT116WT/- cells were transfected with scramble siRNA or with siRNAs specifically designed to target HGS, STRADA, FES, BRAF or PKMYT1. Untransfected cells were also included as a control. Cell density was estimated 72 h after cell transfection by crystal violet staining, and the effect of siRNA on cell proliferation was determined by the ratio ‘O.D. target siRNA’/ ‘O.D. scramble siRNA’. Gray and black rows show the proliferation rate of HCT116WT/S45del and HCT116WT/- cells (relative to scamble), respectively. Note that cell proliferation was affected by the silencing of each genes, both in HCT116WT/S45del and HCT116WT/- cells, except for HGS, the depletion of which affected specifically HCT116WT/S45del cells. (PDF 92 kb