Additional file 4: Figure S4. of Dental follicle stem cells in bone regeneration on titanium implants

DF stem cells cultivated in stem cell medium and osteogenic differentiation medium OS and OC showed that the type of implant do not influence the cell viability and proliferation. Inducing the osteogenic differentiation caused as expected, the decreasing of cell number after 4 and 12 days of cultivation by comparison with DF stem cells cultivated with stem cells medium but with no statistical differences. Both viability assays highlight the mitochondrial and metabolic activity of the cells, but also the tests could be a measurement of cells’ proliferation index. FDA by virtue of its bipolar side chains passively penetrate the cell membrane and is then cleaved non-specifically by intracellular esterases produced by respiratory activity of cells, which convert non-fluorescent FDA to fluorescein. Metabolically active cells with intact membrane can convert also resaurin (the main component of Alamar blue) to resorufin product that can be measured colorimetric or fluorimetric with a microplate reader. Figure S4: Graphical aspect of optical density values (absorbance at 570 nm) of Alamar blue staining of DF stem cells cultivated onto titanium implants in presence of stem cell medium (m stem), simple osteogenic medium (mOS) and complex osteogenic medium (mOC) evaluated after 4 and 12 days (Legend: TiCtrl- Ti6Al7Nb alloy porous titanium, TiHA-titanium infiltrated with hydroxyapatite, TiSiO2-titanium infiltrated with silicatitanate). (PNG 1067 kb