GW3965 treatment did not produce detectable reduction in amyloid beta load.

<p>Amyloid plaque deposition in brains of treated and untreated mice groups were evaluated by immunohistochemistry and soluble Aβ (1–42) by ELISA. <b>(A-B)</b> Representative micrographs of brain sections of Aβ immunohistochemistry with antibody anti-Aβ (6E10). <b>(C-D)</b> No significant differences were observed on the anti-Aβ positive area in the hippocampus or cortex of treated 3xTg-AD mice. Data were expressed as mean ± S.E.M. Statistical analysis was done by one way ANOVA followed by Tukey's multiple comparison test. Females n = 4 per 3xTg-AD group. <b>(E-F)</b> RIPA soluble Aβ42 and Aβ40 in the hippocampus or cortex were measured by ELISA. Quantification data were expressed as mean ± S.E.M. Statistical analysis was done by one way ANOVA followed by Tukey's multiple comparison test. n = 5 per group.