Effect of δ-SG-283R on Localization of β/δ/γ-SG to the Plasma Membrane Surface.

<p>Fig 6<i>A</i> compares cell surface biotinylation assay results between δ-SG-283Q and δ-SG-283R. In HEK293T cells, full-length δ-SG-283Q or δ-SG-283R was separately co-expressed with full-length β-SG and γ-SG. The amount of transfected sarcoglycans targeted to the cell surface was determined by labeling cells with membrane-impermeable NHS-LC-biotin. Biotinylated proteins were isolated with avidin and identified by WB. The specificity of cell surface biotinylation was demonstrated by the absence of cytoplasmic HSP70 in the avidin-bound fraction. In each lane, 10 μg protein was loaded. Fig 6<i>B</i> shows a statistic summary from 5 independent assays. *: <i>p</i><0.05 <i>vs</i> δ-SG-283R.