CAR T cells release IFNγ and Granzyme B in response to antigen.

<p>Culture supernatants were collected 24h after stimulation at day 1 (start) and at endpoint (end). CAR T cells responded to antigen stimulation through secretion of IFNγ (A, B) and Granzyme B (C, D). IFNγ levels were higher in in the groups stimulated with both B cells and IL-2 compared to B cells alone, both at the beginning (3G: p = 0.0418, 2G: p = 0.1389) and at the end of co-culture (3G: p = 0.0228 p = 2G: p = 0.1446). Data was normalized for CAR expression. Error bars represent SEM. Statistical differences were calculated by unpaired t-test with Welch correction.