Quantitative analysis of macropinosome size in fibroblasts co-expressing Rac1^V12 and DGKζ constructs.

<p>(A) Representative images of wild type MEFs transfected with myc-Rac1^V12 alone or cotransfected with the indicated HA-tagged DGKζ construct. Scale bars = 20 um. Magnified images of the boxed regions are shown at the right. Scale bars = 5 um. (B) Graph showing the average macropinosome size in wild type MEFs expressing Rac1^V12 alone (-) or Rac1^V12 and the indicated DGKζ constructs (wt, kd or M1). Values are the mean ± SEM from three independent experiments. Statistical analysis was performed by a one-way ANOVA followed by a Tukey post-hoc multiple comparison test. Asterisks denote a significant difference (p<0.05) between the indicated conditions. (C) Graph showing the cumulative frequency distribution of macropinosome size (um²). The inset shows the distribution of macropinosomes between 0 and 5 um².