Morphological assessment of CE-treated and untreated MCF-7 and MDA-MB231 cells.

<p>A double fluorescent dye (AO/PI) staining method was used on cells after 24 h incubation with the IC₅₀ concentration of CE. Apoptosis characteristics such as, early apoptosis (ap) nuclear compaction (nc), chromatin condensation (cc), the membrane plasma blebbing (mb) and the mitotic cells (mt) were observed in CE-treated (C) MCF-7 and (D) MDA-MB231 cells using an Olympus BX50 fluorescence microscope. Untreated controls (A) MCF-7 and (B) MDA-MB-231 were included. (200 X) magnification morphology.