Effect of the four most common herbal formulas and single herbs on the phosphorylation of myosin light chain (MLC) protein.

<p>Briefly, A10 cells were treated with herbal formulas (A) or single herbs (B). Y27632 (Y10; 10 μM) and calyculin A (A50; 50 μg/ml) were used as negative and positive controls. Western blot analysis and staining with anti-phospho-MLC, anti-total-MLC, and anti-beta actin antibodies was then performed. Phospho-MLC, total-MLC, and beta actin were all obtained with their appropriate protein size bands. The relative Phospho-MLC intensity (%) was expressed as [(Phospho-MLC/total-MLC)_{drug treated}/ (Phospho-MLC/total-MLC)_{cell only} x 100%]. The Mean±SEM values for at least three independent experiments along with the representative western blot were performed.