<p>Sequencing chromatographs are shown for the products from the real-time traditional PCR (top row) and WTB-PCR (bottom row) for three clinical FFPE samples. The numbers at the bottom of each segment indicate the percentages of mutated <i>KRAS</i> allele in the PCR reaction mixtures. The arrows indicate the position of each mutated nucleotide in the <i>KRAS</i> allele.</p
<p>DNA sequencing results of Fas rs1800682 (<b>A</b>), Fas rs2234727 (<b>B</b>), FasL rs763110 (<b>C...
<p>Genomic DNA from FFPE tissues were used for AsP and Mult-AsP PCR assay. In all cases the qPCR ass...
Background: Testing for tumor specific mutations on routine formalin-fixed paraffin-embedded (FFPE) ...
<p>Sequencing chromatographs are shown for the products from the real-time traditional PCR (top row)...
<p>Sequence Chromatograms Displaying the Types of <i>KRAS</i> Mutations Found in This Study</p
<p>A) Electropherogram obtained using Sanger sequencing. The <i>KRAS</i> G12D mutation is identified...
<p>A) Electropherogram obtained using Sanger sequencing. The <i>KRAS</i> Q61H mutation is identified...
<p>(A) Cloning analysis result of Frozen tissues. (B) Cloning analysis result of FFPE tissues.; PCR ...
<p>A) Electropherogram obtained using Sanger sequencing. The mutation is not identified. B) Using AS...
<p>(A) Double peaks labeled with an arrow represented the heterozygous genotype TC (AG). (B) Single ...
<p>Correlation of NGS <i>KRAS</i> mutant allele frequency with digital PCR <i>KRAS</i> mutant allele...
The spotting schema of the barcode sequences is the same of Fig 2A. (A) Cy3 fluorescence image and t...
<p><b>A and B</b>: Corresponding tissue section with areas marked for macrodissection containing ∼70...
<p>Chromatograms on the top demonstrate c.3346C>T, p.Q1116X in <i>UACA</i>. Chromatograms on the bot...
The KRAS mutation status predicts the outcome of treatment with epidermal growth factor receptor tar...
<p>DNA sequencing results of Fas rs1800682 (<b>A</b>), Fas rs2234727 (<b>B</b>), FasL rs763110 (<b>C...
<p>Genomic DNA from FFPE tissues were used for AsP and Mult-AsP PCR assay. In all cases the qPCR ass...
Background: Testing for tumor specific mutations on routine formalin-fixed paraffin-embedded (FFPE) ...
<p>Sequencing chromatographs are shown for the products from the real-time traditional PCR (top row)...
<p>Sequence Chromatograms Displaying the Types of <i>KRAS</i> Mutations Found in This Study</p
<p>A) Electropherogram obtained using Sanger sequencing. The <i>KRAS</i> G12D mutation is identified...
<p>A) Electropherogram obtained using Sanger sequencing. The <i>KRAS</i> Q61H mutation is identified...
<p>(A) Cloning analysis result of Frozen tissues. (B) Cloning analysis result of FFPE tissues.; PCR ...
<p>A) Electropherogram obtained using Sanger sequencing. The mutation is not identified. B) Using AS...
<p>(A) Double peaks labeled with an arrow represented the heterozygous genotype TC (AG). (B) Single ...
<p>Correlation of NGS <i>KRAS</i> mutant allele frequency with digital PCR <i>KRAS</i> mutant allele...
The spotting schema of the barcode sequences is the same of Fig 2A. (A) Cy3 fluorescence image and t...
<p><b>A and B</b>: Corresponding tissue section with areas marked for macrodissection containing ∼70...
<p>Chromatograms on the top demonstrate c.3346C>T, p.Q1116X in <i>UACA</i>. Chromatograms on the bot...
The KRAS mutation status predicts the outcome of treatment with epidermal growth factor receptor tar...
<p>DNA sequencing results of Fas rs1800682 (<b>A</b>), Fas rs2234727 (<b>B</b>), FasL rs763110 (<b>C...
<p>Genomic DNA from FFPE tissues were used for AsP and Mult-AsP PCR assay. In all cases the qPCR ass...
Background: Testing for tumor specific mutations on routine formalin-fixed paraffin-embedded (FFPE) ...