Expression of CD38 and Egr2 protein in M0, M1 and M2 macrophages.

<p>Flow cytometry staining of surface CD38 and intracellular Egr2 (<b>A</b>), CD38 and iNOS (<b>C</b>) or Egr2 and Arginase-1 (<b>E</b>) in BMDM 24 hours post-differentiation in M0, M1 and M2 conditions (representative data of three independent experiments). Flow plots correspond to the CD11b gate. <b>B.</b> Quantification of <b>A</b>, showing the proportion of M0, M1 and M2 macrophages with CD38⁺Egr2^- (putative M1) or CD38^-Egr2⁺ (putative M2) phenotype at 24 hours (n = 3–6 replicates from two independent experiments). <b>D.</b> Quantification of <b>C</b>, showing the proportion of M0, M1 and M2 macrophages with CD38⁺ or iNOS+ phenotype at 24 hours (n = 3 replicates, representative of three independent experiments). <b>F.</b> Quantification of <b>E</b>, showing the proportion of M0, M1 and M2 macrophages with Egr2⁺ or Arg-1⁺ phenotype at 24 hours (n = 3 replicates representative of three independent experiments). Percentage of CD38⁺ cells in iNOS⁺ (<b>G</b>) or TNF-α⁺ (<b>H</b>) BMDM differentiated for 24 hours in M1 conditions. Percentage of Egr2⁺ cells in Arg-1⁺ (<b>I</b>) or CD206⁺ (<b>J</b>) BMDM differentiated for 24 hrs in M2 conditions. **p<0.01, ****p<0.0001.