The integrity of the GPG-motif is dispensable for Nef-induced CD4-downregulation in p56^Lck -negative cells.

<p>A) HeLa-CD4 cells were co-transfected with the wild type or the indicated Nef-GPG mutants (Nef-HA) along with empty or p56^Lck-GFP constructs. Nef activity on CD4-downregulation was analysed by two-colour flow cytometry in GFP cells. A) Histograms represent the percentage of CD4 expression at cell surface relative to non-transfected cells. n.s, not statistically significant. ***, p< 0.001. B) Dot plots and the corresponding MFI are representative of three independent assays.