Mutation of the putative O-glycosylation sites in rhPDGF-BB.

<p>(A) Schematic representation of point mutations in PDGF-BB. (B) Purified wild-type PDGF-BB and PDGF-BB^ΔGly mutant were separated by reducing SDS-PAGE and analyzed by Coomassie Blue staining (left panel), western blotting (middle panel), and glycosylation staining (right panel). A single homogeneous band could be observed after western blotting and Coomassie Blue staining, while no band was detected by glycosylation staining of the mutant.