Purification of recombinant Xf-VapD.
- Juliano S. Mendes (839949)
- André da S. Santiago (839950)
- Marcelo A. S. Toledo (839951)
- Luciana K. Rosselli-Murai (497288)
- Marianna T. P. Favaro (839952)
- Clelton A. Santos (839953)
- Maria Augusta C. Horta (839954)
- Aline Crucello (523961)
- Lilian L. Beloti (839955)
- Fabian Romero (839956)
- Ljubica Tasic (839957)
- Alessandra A. de Souza (449769)
- Anete P. de Souza (838132)
Publication date
January 2016
DOIAbstract
<p>A chromatogram showing the two steps used in the purification of Xf-VapD: Xf-VapD fused with a trx-tag was obtained first, and then trypsin cleavage was used to remove the trx-tag. On the right, a 12.5% SDS-PAGE is shown for the Xf-VapD purification process. The lanes are as follows: M, protein marker; 1, Xf-VapD fused to trx-tag (31 kDa); 2, Xf-VapD and trx-tag after cleavage with trypsin; 3, totally purified Xf-VapD (16.5 kDa); and 4, trx-tag (~13 kDa). The gel was stained using Coomassie brilliant blue.</p
Extracted data
We use cookies to provide a better user experience.