Distribution of Th1, Th2, Th17 and Treg subpopulations out of CD4⁺ T lymphocytes.

<p><b>(A)</b> PBMCs were stained with anti-CD4 PE-cy7, anti-CD25 APC, anti-IFN-γ FITC, anti-IL-17 PE, anti-IL-4 APC and anti-Foxp3 FITC. CD4+ T cells were gated for further analysis. <b>(B)</b> The proportion (%) of IFN-γ⁺/CD4⁺ T cells <b>(C)</b> IL-4⁺/CD4⁺ T cells <b>(D)</b> IL-17⁺/CD4⁺ T cells <b>(E)</b> CD25⁺FOXP3⁺/CD4⁺T cells in each patient group. * <i>P</i><0.05 for each comparison. LTS, long term stable; CAD, chronic allograft dysfunction; ES, early stable; ESRD, end stage renal disease; HC, healthy control.