Effects of hydrostatic pressure on cell death.

<p>(A) Phase-contrast microscopic images of the culture supernatant of MDCK I cells cultured on filters for four days. Many spherical floating objects were observed. Scale bars = 100 μm for left panel and 20 μm for right panel. (B) A transmission electron micrograph of the culture supernatant of MDCK I cells. Scale bar = 1 μm. (C) Volume of the debris in the culture supernatant. The volume of the debris was measured as described in <i>Materials and Methods</i>. The volume of debris in days 6–8 under the ‘Basal’ condition was significantly smaller than that under the ‘Apical’ condition in MDCK I cells. ** <i>p</i> < 0.01 compared with the ‘Apical’ condition at corresponding day.