Effects of signal inhibitors and activators on the epithelial stratification by the hydrostatic pressure.

<p>(A) Light microscopic images in a vertical section under the ‘Apical’ condition in the presence of the signal inhibitor or activator in MDCK I cells. The upper side is apical side and the lower side is basal side. (B) Stratification index of MDCK I cells under the ‘Apical’ condition in the presence of the signal inhibitor or activator. The stratification index in the presence of H89 and SQ22536 was significantly higher than that in the control experiment (DMSO). (C) Light microscopic images in a vertical section under the ‘Basal’ condition in the presence of the signal inhibitor or activator in MDCK I cells. The upper side is apical side and the lower side is basal side. (D) Stratification index of MDCK I cells under the ‘Basal’ condition in the presence of the signal inhibitor or activator. The administration of H89 significantly increased the degree of epithelial stratification by the hydrostatic pressure, and the administration of forskolin significantly decreased the degree of the epithelial stratification. ** <i>p</i> < 0.01 compared with control (DMSO).