Auxin and ethylene responsiveness of <i>SlARF2A</i> and <i>SlARF2B</i> genes.

<p>(A) qPCR analysis of <i>SlARF2A</i> and <i>SlARF2B</i> transcripts in total RNA samples extracted from wild-type mature green fruits treated with 50 ml.L^-1 ethylene for 5 hours. (B) qPCR analysis of <i>SlARF2A</i> and <i>SlARF2B</i> transcripts in total RNA samples extracted from wild-type breaker fruits treated with 1-MCP (1.0 mg.L^-1) for 16 hours. (C) qPCR analysis of <i>SlARF2A</i> and <i>SlARF2B</i> transcripts in total RNA samples extracted from wild-type mature green fruits treated with 20 μM IAA or buffer (control) for 6 hours. The relative mRNA levels of <i>SlARF2A</i>/<i>SlARF2B</i> genes were normalized against actin. The results were expressed using control untreated fruit as reference with relative mRNA level set to 1. Error bars mean ±SD of three biological replicates. Stars indicate the statistical significance using Student’s t-test: *0.01 < p-value < 0.05, ** 0.001< p-value <0.01, *** p-value < 0.001. <i>E4</i>, <i>E8</i>: ethylene response genes; <i>GH3</i>, <i>SAUR</i>: auxin response genes.