AI/MT-induced masculinization with a reversible sex change after the termination of orally administered AI/MT in orange-spotted grouper.

<p>(A) The sampling program during sexual fate alternation from female to male or vice versa, including fish collected before oral administration, and 3 mo (T, the treatment period, 7- to 10-mo-old) after AI/MT-oral administration, and after 3 wks (T+3w), 5 wks (T+5w) and 9 wks (T+9w) of AI/MT-termination. The gonadal status is shown as follows: (B) Initial control female; (C) AI-induced male with active spermatogenesis 3 mo after AI-oral administration; (D), (E), and (F) Regenerated female 3 wks, 5 wks, and 9 wks after AI-termination, respectively; (G) MT-induced male with active spermatogenesis 3 mo after MT-oral administration; (H), (I), and (J) Regenerated female after 3 wks, 5 wks, and 9 wks of MT-termination, respectively. L, central lumen; OG, oogonia; PO, primary oocyte; SG, spermatogonia; SC, spermatocyte; SZ, spermatozoa; EG, early germ cell.