Immunohistochemical analysis of α-sma expression in liver section.

<p>(A) Representative images of smooth muscle actin immunohistochemical staining in liver. (B) The expression of α-sma in losartan-HA micelle group is significantly lower than those of the HA micelle and losartan groups. The proportion of areas immunopositive for smooth muscle actin was measured using IHC profiler plugin in ImageJ (NIH image program, version 1.49) software as mean percent positive areas. The data are presented as the mean ± SD *P <0.001 and ^#P <0.001 relative to HA micelle and losartan group respectively.