Inhibition of proliferation in RCC cells by hnRNP K knocking down.

<p>Expression of heterogeneous nuclear ribonucleoprotein (hnRNP) K in both normal renal proximal tubule cell line (RPTEC) and renal cell carcinoma (RCC) cell lines, and growth inhibition of four RCC cell lines after knock-down of endogenous hnRNP K. As described in the Materials and Methods section, the number of viable RCC cells after treatment with control siRNA or siRNA against hnRNP K (si-hnRNP K) for 0, 24, 48, 72, 96 or 120 h was determined by water-soluble tetrazolium salt (WST-1) assay. A. Total cell lysates were prepared from each cell line and analyzed by western blotting using anti-hnRNP K antibody to detect hnRNP K protein (upper panel). To elucidate equal loaded amounts of total cell lysates, western blotting using anti-β-actin antibody was also performed (lower panel); B. Each RCC cell line transfected with negative control siRNA or si-hnRNP K was harvested 72 h after transfection and total cell lysates were analyzed by western blotting using anti-hnRNP K antibody to evaluate the efficacy of si-hnRNP K; C. Values measured at 0 h with control siRNA or si-hnRNP K were determined as 1. Three individual experiments were performed. Results were presented as mean ± S.D. *<i>P</i><0.05 compared with control (Student’s <i>t</i>-test, n = 3).