Effects of HX630 on corticotroph tumor cells <i>in vivo</i>.

<p>The mice (nu/nu) were subcutaneously inoculated with AtT20 cells (1,000,000/mouse). After 1 week, they were randomized for injection with either HX630 (5 mg/kg/d) or vehicle (corn oil) intraperitonealy 3 times a week for 3 weeks. In A and B, the body weights and the tumor volumes were monitored throughout the treatment. In C and D, a representative mouse of either the control group or the HX630-treated group on the last day is shown, respectively. Thereafter, mice were euthanized and their tumors were removed. Total RNAs were isolated from tumors and were subjected to examine <i>Pomc</i> mRNA expression by real-time PCR. In E and F, tumor volumes and <i>Pomc</i> mRNA expression in tumor cells on the last day were compared between the control group and the HX630 treated group, respectively. Results of <i>Pomc</i> mRNA expression are expressed as percentages of the control. Each point represents mean ± SEM (n = 7). *<i>P</i><0.05 vs control.