Aberrant lymph nodes in <i>Rras</i>^−/− mice.

<p><b>(</b>A) Immunohistochemical analysis of <i>Rras</i>^<b>+/+</b> (WT) and <i>Rras</i>^−/− (KO) lymph nodes stained with anti-MECA-79 (<i>brown</i>). Magnified areas (<i>boxed</i>) are shown in lower panels. <i>Bars</i>, 200 μm. (B) Staining intensity was quantified from five lymph nodes. <i>Bars</i>, means. ***<i>p</i><0.0001, <i>t</i>-test. (C) Surface expression of CD62L in CD4^<b>+</b> and CD8^<b>+</b> T cells from spleen and lymph nodes (LN) of <i>Rras</i>^<b>+/+</b> and <i>Rras</i>^−/− mice was analyzed with flow cytometry. Isotype immunoglobulin (<i>Ig</i>) was used as a control. (D) The results from six mice per group were quantified and depicted. <i>Bars</i>, SD, two-tailed <i>t</i>-test. **<i>p</i><0.005, ***<i>p</i><0.0001.