Additional file 3: Figure S3. of Post-CNS-inflammation expression of CXCL12 promotes the endogenous myelin/neuronal repair capacity following spontaneous recovery from multiple sclerosis-like disease

In vitro differentiation of aNSCs isolated from the subventricular zone of adult mice. Neurospheres that were expanded in neurosphere medium in the presence of growth factors were differentiated on PDL-coated coverslips in the presence of 5 % FCS in the absence of growth factors. On day 0, a very small proportion of cells in neurospheres were NPCs (DCX+), but none were OPCs (NG2+), maturing neuronal cells (NF200+), or oligodendrocytes (MBP+). The differentiation cultures were fixed after 2, 4, and 8 days and immunostained for indicated markers of neuronal, oligodendroglial, or astrocytic lineages. At day 2 of culture, differentiation to NPCs (DCX+), OPCs (NG2+), and astrocytes (GFAP+) were observed, which matured with time (day 4 and 8) to neuronal cells (β-tubulin III+), MBP-producing oligodendrocytes, or to astrocytes. Nuclei were visualized by DAPI counterstaining. Scale bar: 50 μm. (TIF 8316 kb