Additional file 3: of IL-4 orchestrates STAT6-mediated DNA demethylation leading to dendritic cell differentiation

(A) Scatterplots showing DNA methylation profiles of matching pairs (MO-iDC; MO-iMAC; iDC-mDC; iMAC-mMAC). CpGs with significant differences (>2-fold change or <0.5-fold change; p < 0.01 and FDR < 0.05) in average results for three samples are highlighted in blue (DCs) or red (MACs). The x-axis and y-axis of each graph correspond to the β values for each paired comparison, as indicated in the lower left corner. (B) A Venn diagram showing the overlap between Zhang et al. [17] data and our own data corresponding to the list of demethylated genes in MO-to-iDC differentiation. (C) Technical validation of the array data by bisulfite pyrosequencing of modified DNA. Three groups of genes are represented: demethylated genes specific to iDC differentiation, demethylated genes specific to iMAC differentiation, and genes that are commonly demethylated in iDC and iMAC differentiation. (D) 5hmC content in several of the CpGs that are rapidly demethylated after cytokine addition to MOs. (PDF 1037 kb